Germline mutagenesis of Nasonia vitripennis through ovarian delivery of CRISPR‐Cas9 ribonucleoprotein
نویسندگان
چکیده
منابع مشابه
Function and Evolution of DNA Methylation in Nasonia vitripennis
The parasitoid wasp Nasonia vitripennis is an emerging genetic model for functional analysis of DNA methylation. Here, we characterize genome-wide methylation at a base-pair resolution, and compare these results to gene expression across five developmental stages and to methylation patterns reported in other insects. An accurate assessment of DNA methylation across the genome is accomplished us...
متن کاملReciprocal inheritance of centrosomes in the parthenogenetic Hymenopteran Nasonia vitripennis
BACKGROUND In the majority of animals, the centrosome-the microtubule-organizing center of the cell-is assembled from components of both the sperm and the egg. How the males of the insect order Hymenoptera acquire centrosomes is a mystery, as they originate from virgin birth. RESULTS To address this issue, we observed centrosome, spindle and nuclear behavior in real time during early developm...
متن کاملPatterning the dorsal-ventral axis of the wasp Nasonia vitripennis.
Regulatory networks composed of interacting genes are responsible for pattern formation and cell type specification in a wide variety of developmental contexts. Evolution must act on these regulatory networks in order to change the proportions, distribution, and characteristics of specified cells. Thus, understanding how these networks operate in homologous systems across multiple levels of phy...
متن کاملInheritance of gynandromorphism in the parasitic wasp Nasonia vitripennis.
The parasitic wasp Nasonia vitripennis has haplo-diploid sex determination. Males develop from unfertilized eggs and are haploid, whereas females develop from fertilized eggs and are diploid. Females and males can be easily distinguished by their morphology. A strain that produces individuals with both male and female features (gynandromorphs) is studied. We provide data on female/male patterni...
متن کاملWaspAtlas: a Nasonia vitripennis gene database and analysis platform
Nasonia vitripennis is a parasitoid wasp which is becoming an important model organism for parasitism, epigenetics, evolutionary and developmental genetics. WaspAtlas is a new gene database in which we have compiled annotation data from all available N. vitripennis releases along with a wealth of transcriptomic data, methylation data and original analyses and annotations to form a comprehensive...
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ژورنال
عنوان ژورنال: Insect Molecular Biology
سال: 2020
ISSN: 0962-1075,1365-2583
DOI: 10.1111/imb.12663